Description
The BD™ CBA Mouse Th1/Th2 Cyokine Kit (Catalog No. 551287) can be used to measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interferon-γ (IFN-γ), and Tumor Necrosis Factor (TNF) protein levels in a single sample. The kit performance has been optimized for analysis of physiologically relevant concentrations (pg/mL levels) of specific cytokine proteins in tissue culture supernatants and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples. Vial Label Reagent Quantity A1 Mouse IL-2 Capture Beads 1 vial, 0.8 mL A2 Mouse IL-4 Capture Beads 1 vial, 0.8 mL A3 Mouse IL-5 Capture Beads 1 vial, 0.8 mL A4 Mouse IFN-γ Capture Beads 1 vial, 0.8 mL A5 Mouse TNF Capture Beads 1 vial, 0.8 mL B Mouse Th1/Th2 PE Detection Reagent 1 vial, 4 mL C Mouse Th1/Th2 Cytokine Standards 2 vials lyophilized D Cytometer Setup Beads 1 vial, 1.5 mL E PE Positive Control Detector 1 vial, 0.5 mL E2 FITC Positive Control Detector 1 vial, 0.5 mL F Wash Buffer 1 bottle, 130 mL G Assay Diluent 1 bottle, 30 mL
Preparation And Storage
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Mouse IL-2 Capture Beads | 0.8 mL (1 ea) | 51-9005801 | N/A |
| Mouse IL-4 Capture Beads | 0.8 mL (1 ea) | 51-9005803 | N/A |
| Mouse IL-5 Capture Beads | 0.8 mL (1 ea) | 51-9005805 | N/A |
| Mouse IFN-γ Capture Beads | 0.8 mL (1 ea) | 51-9005807 | N/A |
| Mouse TNF Capture Beads | 0.8 mL (1 ea) | 51-9005809 | N/A |
| B) Mouse Th1/Th2 PE Detection Reagent | N/A | 51-2523KC | N/A |
| Mouse Th1/Th2 Cytokine Standards | N/A | 51-2524KC | N/A |
| D Cytometer Setup Beads | 1 Each (1 ea) | 51-9005731 | N/A |
| E1 PE Positive Control Detector | 1 Each (1 ea) | 51-9005732 | N/A |
| E2 FITC Positive Control Detector | 1 Each (1 ea) | 51-9005734 | N/A |
| F Wash Buffer | 1 Each (1 ea) | 51-2431KC | N/A |
| G Assay Diluent | 1 Each (1 ea) | 51-2432KC | N/A |
Development References (13)
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Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). View Reference
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McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). View Reference
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Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
