Purified Mouse Anti-Mouse I-E[k]
Clone 14-4-4S (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Mouse C3H.SW IgG2a, κ
- Reactivity Mouse (QC Testing) Rat (Reported)
Flow cytometry (Routinely Tested)
Blocking, Induction, Cytotoxicity, Immunoprecipitation (Reported)
- Immunogen C3H mouse skin graft and splenocytes
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 14-4-4S monoclonal antibody specifically recognizes the I-E[k] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[r] haplotypes. Cells from mice of the H-2[b], H-2[f], H-2[g7], H-2[q], and H-2[s] haplotypes do not express I-E antigen. It has been reported that mAb 14-4-4S crossreacts with the rat MHC class II antigen RT1D.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.