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      Purified Mouse Anti-Mouse I-E[k]
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing), Rat (Reported)
      Mouse C3H.SW IgG2a, κ
      C3H mouse skin graft and splenocytes
      Flow cytometry (Routinely Tested), Blocking, Cytotoxicity, Immunoprecipitation, Induction (Reported)
      0.5 mg/ml
      AB_397093
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      558734 Rev. 10
      Antibody Details
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      14-4-4S

      The 14-4-4S monoclonal antibody specifically recognizes the I-E[k] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[r] haplotypes. Cells from mice of the H-2[b], H-2[f], H-2[g7], H-2[q], and H-2[s] haplotypes do not express I-E antigen. It has been reported that mAb 14-4-4S crossreacts with the rat MHC class II antigen RT1D.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      558734 Rev. 10
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558734 Rev.10
      Citations & References
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      Development References (10)

      1. Bhattacharya A, Dorf ME, Springer TA. A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981; 127(6):2488-2495. (Clone-specific: Immunoprecipitation). View Reference
      2. Blankenhorn EP, Symington FW, Cramer DV. Biochemical characterization of Ia antigens encoded by the RT1.B and RT1.D loci in the rat MHC. Immunogenetics. 1983; 17(5):475-484. (Clone-specific). View Reference
      3. Grakoui A, Bromley SK, Sumen C, et al. The immunological synapse: a molecular machine controlling T cell activation. Science. 1999; 285(5425):221-227. (Clone-specific: Immunoaffinity chromatography). View Reference
      4. Harton JA, Litaker W, Frelinger JA, Bishop GA. Structure function analysis of the H-2 Abp gene. Immunogenetics. 1991; 34(6):358-365. (Clone-specific: Induction). View Reference
      5. Hattori M, Buse JB, Jackson RA, et al. The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex. Science. 1986; 231(4739):733-735. (Biology). View Reference
      6. Klein J. Mutations in H-2E loci. In: Klein J. Natural History of the Major Histocompatibility Complex. New York: John Wiley & Sons; 1986:216-218.
      7. Lu L, Woo J, Rao AS, et al. Propagation of dendritic cell progenitors from normal mouse liver using granulocyte/macrophage colony-stimulating factor and their maturational development in the presence of type-1 collagen. J Exp Med. 1994; 179(6):1823-1834. (Clone-specific: Cytotoxicity). View Reference
      8. Neiss U, Reske K. Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture. Int Immunol. 1994; 6(1):61-71. (Clone-specific: Immunoprecipitation). View Reference
      9. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980; 124(2):533-540. (Immunogen: Cytotoxicity). View Reference
      10. Roy M, Aruffo A, Ledbetter J, Linsley P, Kehry M, Noelle R. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol. 1995; 25(2):596-603. (Clone-specific: Blocking). View Reference
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      558734 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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