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      APC Rat Anti-Mouse CD70
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      APC Rat Anti-Mouse CD70
      Flow cytometric analysis of CD70 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide (LPS; Sigma L-8272; 1 μg/ml) for 3 days. The activated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat IgG2b, κ Isotype Control (Cat. No. 556924; dashed line histogram) or APC Rat Anti-Mouse CD70 antibody (Cat. No. 563634; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      APC Rat Anti-Mouse CD70
      Flow cytometric analysis of CD70 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide (LPS; Sigma L-8272; 1 μg/ml) for 3 days. The activated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat IgG2b, κ Isotype Control (Cat. No. 556924; dashed line histogram) or APC Rat Anti-Mouse CD70 antibody (Cat. No. 563634; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      CD27 ligand; CD27l, CD27LG; Tnfsf7; Ki-24 Antigen
      Mouse (QC Testing)
      Rat F344, also known as Fischer, CDF IgG2b, κ
      BALB/c mouse B lymphoma A20.2J
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2738336
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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      563634 Rev. 1
      Antibody Details
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      FR70

      The FR70 monoclonal antibody specifically binds to mouse CD70, a 30-33-kDa type-II transmembrane glycoprotein belonging to the TNF/NGF superfamily. CD70 is the ligand for CD27 and is expressed primarily on activated B cells. CD70 expression can be induced by activation of splenocytes with lipopolysaccharide and stimulatory antibodies specific for IgM, CD40 or both. mRNA coding for CD70 is transiently expressed after Concanavalin A stimulation of splenocytes or thymocytes. CD70 has been observed to be expressed on dendritic cells of Leishmania major-infected mice. The CD27-CD70 interaction delivers costimulatory signals to T cells and NK cells. It has been reported that FR70 antibody blocks binding of mCD27-Ig fusion protein to mouse Cd70-transfected cells and inhibits some in vitro T-cell- and NK-cell-dependent responses.

      563634 Rev. 1
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      563634 Rev.1
      Citations & References
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      Development References (5)

      1. Akiba H, Miyahira Y, Atsuta M. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med. 2000; 191(2):375-382. (Clone-specific: Flow cytometry, In vivo exacerbation, Neutralization). View Reference
      2. Hartwig UF, Karlsson L, Peterson PA, Webb SR. CD40 and IL-4 regulate murine CD27L expression. J Immunol. 1997; 159(12):6000-6008. (Clone-specific: Neutralization). View Reference
      3. Oshima H, Nakano H, Nohara C. Characterization of murine CD70 by molecular cloning and mAb. Int Immunol. 1998; 10(4):517-526. (Immunogen: Blocking, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Neutralization). View Reference
      4. Takeda K, Oshima H, Hayakawa Y, et al. CD27-mediated activation of murine NK cells. J Immunol. 2000; 164(4):1741-1745. (Clone-specific: Functional assay, Inhibition). View Reference
      5. Tesselaar K, Gravestein LA, van Schijndel GM, Borst J, van Lier RA. Characterization of murine CD70, the ligand of the TNF receptor family member CD27. J Immunol. 1997; 159(10):4959-4965. (Biology). View Reference
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      563634 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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