Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The N71-1183 monoclonal antibody specifically recognizes the alpha subunit, also known as p19, of mouse interleukin 23 that is encoded by the Il23a gene. Interleukin 23 (IL-23) is a heterodimeric cytokine composed of a p40 subunit and a p19 subunit. The p19 subunit is unique to IL-23, while the p40 subunit is shared with IL-12 and is also known as IL-12B. Human and mouse p19 share 70% amino acid sequence identity. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4 and TLR8 ligands, as well as the β-glucan receptor, Dectin-1. IL-23 has biological activities that are similar to, but distinct from IL-12: Both IL12 and IL-23 induce proliferation and IFN-γ production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 are restricted to memory T cells. IL-23, but not IL-12, has been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. In vitro and in vivo treatment with N71-1183 monoclonal antibody has been shown to inhibit IL-23's ability to enhance IL-17 production by activated MRL/lpr T lymphocytes (Kyttaris VC, Kampagianni O, Tsokos GC, 2013).
Development References (2)
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Kyttaris VC, Kampagianni O, Tsokos GC. Treatment with Anti-Interleukin 23 Antibody Ameliorates Disease in Lupus-Prone Mice.. Biomed Res Int. 2013; 2013Available: http://www.ncbi.nlm.nih.gov/pubmed/23841097 2014, December 3. View Reference
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Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
