Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Brilliant Violet™ 510 is a trademark of Sirigen.
Companion Products
The 5B5 monoclonal antibody specifically binds to human CD77, a small neutral glycosphingolipid also referred to as globotriaosylceramide (Gb3), Burkitt 's lymphoma associated antigen (BLA), ceramide trihexoside (CTH), and Pk blood group antigen. CD77 is expressed on a subset of germinal center B-lymphocytes including centroblasts that are found with greater frequency in tonsil and mucosal lymphoid tissues and less frequently in peripheral blood, bone marrow and spleen. It has been described as a receptor for Shiga Toxin, produced by Shigella dysenteriae, and Verotoxin 1 (VT1), produced by some strains of E. coli. CD19 has been reported as a ligand for CD77. This interaction may function in adhesion and signal transduction at a specific stage of B cell differentiation. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
Development References (5)
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Fyfe G, Cebra-Thomas JA, Mustain E, Davie JM, Alley CD, Nahm MH. Subpopulations of B lymphocytes in germinal centers. J Immunol. 1987; 139(7):2187-2194. (Immunogen: Flow cytometry, Immunofluorescence, Radioimmunoassay). View Reference
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Lagresle C, Bella C, Daniel PT, Krammer PH, Defrance T. Regulation of germinal center B cell differentiation. Role of the human APO-1/Fas (CD95) molecule. J Immunol. 1995; 154(11):5746-5756. (Biology). View Reference
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Maloney MD, Lingwood CA. CD19 has a potential CD77 (globotriaosyl ceramide)-binding site with sequence similarity to verotoxin B-subunits: implications of molecular mimicry for B cell adhesion and enterohemorrhagic Escherichia coli pathogenesis. J Exp Med. 1994; 180(1):191-201. (Biology). View Reference
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Mangeney M, Rousselet G, Taga S, Tursz T, Wiels J. The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis. Mol Immunol. 1995; 32(5):333-339. (Biology). View Reference
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Nahm MH, Takes PA, Bowen MB, Macke KA. Subpopulations of B lymphocytes in germinal centers, II. A germinal center B cell subpopulation expresses sIgD and CD23. Immunol Lett. 1989; 21(3):201-208. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
