APC Mouse Anti-Human CD79b
Clone 3A2-2E7 (also known as SN8) (RUO (GMP))
- Concentration 100 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Cell membranes from human B-prol ymphocytic leukemia (B-PLL) cells
- Workshop No. V B037
- Entrez Gene ID 974
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
The CD79b antibody, clone SN8 (3A2-2E7-1F5), is generated from the hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with cell membrane preparations from B-prolymphocytic leukemia (B-PLL) cells.
The CD79b antibody recognizes an epitope on the extracellular domain of a 36–40 kilodalton (kDa) type I membrane glycoprotein. Immunoglobulin (Ig) antigen receptors are composed of a non-covalently associated complex of Ig and two other proteins, Igα and Igβ, which have been designated as CD79a and CD79b, respectively.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.