Alexa Fluor® 647 Mouse anti-Human CD191

BD Pharmingen™ Alexa Fluor® 647 Mouse anti-Human CD191

Name: Alexa Fluor® 647 Mouse anti-Human CD191
Brand: BD Pharmingen™
SKU: 557914

Clone 53504

(RUO)

Flow cytometric analysis of Alexa Fluor®-647 conjugated anti-human CD191 on human peripheral monocytes. Human peripheral leukocytes were stained with Alexa Fluor® 647-conjugated antihuman CD191 antibody (Clone 53504, Cat. No. 557914; open histogram) or Alexa Fluor® 647-conjugated mouse IgG2b (Clone 27-35, please inquire; shaded histogram). Flow cytometry was performed and histograms were derived from gated events based on light scattering characteristics of viable monocytes.

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD Pharmingen™

Alternative Name: CCR1; C-C CKR-1; C-C chemokine receptor type 1; SCYAR1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2b

Immunogen: Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Workshop Number: VIII 80128

RRID: AB_2074252

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Alexa Fluor® is a registered trademark of Life Technologies Corporation.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.

557914 Rev. 1

Antibody Details

53504

CD191 (also known as CCR1, CC-CKR1, HM145 and MIP-1αR), a seven-transmembrane, G-proteincoupled receptor, is the specific receptor for CC chemokine CCL3, CCL5, CCL7, CCL8, CCL14, CCL15, CCL16 and CCL23. It has been reported that human CD191 is expressed in peripheral monocytes and lymphocytes, bone marrow CD34+ cells. In addition, CD191 mRNA has been detected in dendritic cells and eosinophils. The human CD191 gene has been mapped to chromosome 3p21. The immunogen used to generate 53504 hybridoma was human CD191-transfected mouse cells. The antibody does not cross-react with human CCR5 transfected cells.

557914 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

557914 Rev.1

Citations & References

Development References (4)

Gao, J.L., D. B. Kuhns, et al. Structure and functional expression of the human macrophage inflammatory protein 1a/RANTES receptor.. 1993; 177(5):1421-1427. (Biology). View Reference
Neote, K., D. DiGregorio, J. Y. Mak, R. Horuk, and T. J. Schall. Molecular cloning, functional expression and signaling characteristics of a C-C chemokine receptor.. 1993; 72(3):415-425. (Biology). View Reference
Saeki T, Naya A.. CCR1 chemokine receptor antagonist.. 2003; 9(15):1201-1208. (Biology). View Reference
Su, S. B., N. Mukaida, J. Wang, H. Nombra, and K. Matsushima. Perparation of specific polyclonal antibodies to a C-C chemokine receptor, CCR1, and determination of CCR1 expression on various types of leukocytes.. 1996; 60(5):658-666. (Biology). View Reference

View All (4)

557914 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.