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Alexa Fluor® 700 Rat Anti-Mouse Ig, κ Light Chain
Alexa Fluor® 700 Rat Anti-Mouse Ig, κ Light Chain
Flow cytometric analysis of mouse Ig, κ light chain expression on mouse splenocytes.  Splenocytes from BALB/c mice were stained with PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with an Alexa Fluor® 700 Rat IgG1, κ Isotype Control (Cat. No. 558001, Left Panel) or with the Alexa Fluor® 700 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561351, Right Panel).  Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of mouse Ig, κ light chain expression on mouse splenocytes.  Splenocytes from BALB/c mice were stained with PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with an Alexa Fluor® 700 Rat IgG1, κ Isotype Control (Cat. No. 558001, Left Panel) or with the Alexa Fluor® 700 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561351, Right Panel).  Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Product Details
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BD Pharmingen™
Ig kappa chain C region; IGKC; Igk-C; Ig, κ
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Flow cytometry (Routinely Tested)
0.2 mg/ml
16071
AB_10611851
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
561351 Rev. 1
Antibody Details
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187.1

The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.

561351 Rev. 1
Format Details
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Alexa Fluor™ 700
Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 700
Red 627-640 nm
697 nm
719 nm
561351 Rev.1
Citations & References
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Development References (1)

  1. Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference
561351 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.