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Purified Hamster IgG1, κ Isotype Control RUO

Purified Hamster IgG1, κ Isotype Control 

Clone  A19-3   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Anti-Trinitrophenol (TNP); TNP (Isotype Control Arm)
  • Concentration 0.5 mg/ml
  • Isotype Armenian Hamster IgG1, κ
  • Application ELISA (Routinely Tested) 
    Isotype control, Flow cytometry (Tested During Development) 
  • Immunogen TNP-keyhole limpet hemocyanin
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The A19-3 monoclonal antibody specifically binds to the hapten, trinitrophenol (TNP). TNP is not expressed on human, mouse, or rat cells. The immunoglobulin from clone A19-3 was selected as an isotype control following screening for low background staining on a variety of mouse and human tissues.

Format
  • Format Purified

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Suggested Companion Products

Purified Hamster IgG1, κ Isotype Control RUO
1 mL Cat No: 550344

Resources & Tools
 Spectrum Viewer  Download TDS  Download MSDS
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). For immunohistochemical staining, we recommend the use of purified A19-3 mAb in our special formulation for immunohistochemistry, Cat. No. 550344.

553969 Rev. 13
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