Purified Mouse Anti-TRADD
Clone 37/TRADD (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name TNFR1-Associated Death Domain protein
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing) Rat (Tested in Development)
Western blot (Routinely Tested)
Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
- Immunogen Human TRADD aa. 163-312
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
TNFα (Tumor Necrosis Factor) stimulates programmed cell death and NF-κB activation as a result of its binding to the TNF receptor 1 (TNFR1). Within this receptor, a sequence referred to as the "death domain" has been shown to be necessary for both of these functions. Using the yeast two-hybrid system to detect proteins which interact with the receptor through this "death domain", a 34 kDa protein was found and designated TRADD (TNFR1-Associated Death Domain protein). TRADD appears to contain no intrinsic catalytic activity. It also contains a death domain and it has been shown to bind to FADD and RIP. Mutational analysis of TRADD demonstrates that programmed cell death and NF-κB activation are distinct and controlled independently.
- Format Purified
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at -20°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml