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Purified Mouse Anti-RIP 38/RIP RUO

Purified Mouse Anti-RIP 

Clone  38/RIP   (RUO)

  • Brand BD Transduction Laboratories™
  • Alternative Name Receptor Interacting Protein
  • Concentration 250 µg/ml
  • Isotype Mouse IgG2a
  • Reactivity Human (QC Testing)  Mouse, Rat, Dog, Chicken (Tested in Development) 
  • Application Western blot (Routinely Tested) 
    Immunofluorescence, Immunoprecipitation (Tested During Development) 
    Immunohistochemistry (Not Recommended) 
  • Immunogen Human RIP aa. 385-650
  • Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

Binding or cross linking of the Fas antigen (also known as APO-1 and CD95) is known to elicit apoptosis in susceptible cells. Fas is a member of a family of cell surface receptors which includes tumor necrosis factor receptors (TNF-R, and TNF-R2) and nerve growth factor receptors (NGF-R), CD40, OX40, CD30, CD27, and 4-1BB. Several members of this family have been shown to regulate or induce cell death (TNF-R1 and TNF-R2). A 74 kDa member of this family protein named RIP (Receptor Interacting Protein) contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic "death domain" present in both Fas and TNF-R1. Both Fas and RIP have been shown to require this death domain to induce apoptosis and overexpression of RIP has been shown to induce cell death in transfected cells.

Format
  • Format Purified
Suggested Companion Products

Human Endothelial Cell Lysate RUO
500 µg Cat No: 611450

HRP Goat Anti-Mouse Ig RUO
1 mL Cat No: 554002

FITC Goat Anti-Mouse Ig Polyclonal RUO
0.5 mg Cat No: 554001

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.


Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml


  1. Devin A, Lin Y, Yamaoka S, Li Z, Karin M, Liu Zg. The alpha and beta subunits of IkappaB kinase (IKK) mediate TRAF2-dependent IKK recruitment to tumor necrosis factor (TNF) receptor 1 in response to TNF. Mol Cell Biol. 2002; 21(12):3986-3994. View reference

  2. Fulda S, Meyer E, Debatin KM. Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression. Cancer Res. 2000; 60(14):3947-3956. View reference

  3. Lewis J, Devin A, Miller A, et al. Disruption of hsp90 function results in degradation of the death domain kinase, receptor-interacting protein (RIP), and blockage of tumor necrosis factor-induced nuclear factor-kappaB activation. J Biol Chem. 2000; 275(14):10519-10526. View reference

  4. Stanger BZ, Leder P, Lee TH, Kim E, Seed B. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell. 1995; 81(4):513-523. View reference

  5. Takahashi T, Tanaka M, Brannan CI, et al. Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand. Cell. 1994; 76(6):969-976. View reference

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