Purified Mouse Anti-Human Retinoblastoma Protein
Clone XZ91 (RUO)
- Brand BD Pharmingen™
- Alternative Name RB
- Concentration 0.5 mg/ml
- Isotype Mouse IgG2a
- Reactivity Human (QC Testing) Chicken, Mink (Reported)
Western blot (Routinely Tested)
Immunofluorescence, Immunoprecipitation (Reported)
- Immunogen Recombinant carboxy-terminal truncated Rb protein
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as a tumor suppressor gene product. An underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the hyperphosphorylated form is present in proliferating cells. Only the underphosphorylated form of Rb binds specifically to viral oncogenes such as SV40 large T, adenoviral E1A and HPV-E7. This interaction may partially contribute to the transforming activity of these viral oncoproteins. Rb also interacts with several cyclins including A, D and E, as well as the transcriptional activator E2F. The importance of these interactions for the biological function of Rb is still being elucidated.
Clone XZ91 recognizes an epitope located between amino acids 444-535 of human Rb. XZ91 cross reacts with chicken and mink Rb. A recombinant carboxy-terminal truncated Rb protein (containing approximately 60 kD of the Rb coding region) was used as immunogen.
- Format Purified
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Applications include immunoprecipitation (1-2 µg/one million cells), western blot analysis (2 µg/ml) and immunofluorescence microscopy of cultured cells. Rb migrates as multiple closely-spaced bands between approximately 110-116 kD when sized on denaturing polyacrylamide gels (i.e. by SDS-PAGE). The different bands represent different Rb phosphorylation states; the higher molecular weight bands are more highly phosphorylated than the lower molecular weight bands. The level of phosphorylation is cell cycle dependent, and may also be cell type dependent (not all forms are seen in all cell types that express Rb). Polyacrylamide gel conditions influence the actual number of bands observed. In cases where optimal band separation is desired, we recommend a 4 to 20% gradient gel (≥ 12 inches long). MOLT-4 human leukemia cells (ATCC CRL-1582) are suggested as a positive control.