Purified Mouse Anti-Human Caspase-9
Clone B40 (RUO)
- Brand BD Pharmingen™
- Alternative Name ICE-LAP-6, Mch6, Apaf-3
- Concentration 0.5 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Western blot (Routinely Tested)
- Immunogen Human Caspase-9 aa. 364-405 Recombinant Protein
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Caspase 9 (ICE-LAP6, Mch-6, Apaf-3) is a member of a family of cysteine proteases which play a critical role in the induction of programmed cell death. Members of this family have been grouped according to sequence homology as being either ICE-like proteases (caspases 1, 4 and 5) or Ced-3- like proteases (caspases 3, 6, 7, 9 and 10a). Caspase-9 is produced as a 48 kD precursor (procaspase-9) which contains an N-terminal prodomain with high homology to caspase-2. Procaspase-9 may be processed into subunits which heterodimerize to form the active enzyme. Activation of caspase-9 occurs in the presence of cytochrome c (Apaf-2), following an interaction between caspase-9 and Apaf-1. Activation may also be triggered directly by the cytotoxic T-cell protease, granzyme B. Active caspase-9 cleaves and thus activates caspase-3 and is also a relevant target of active caspase-3. Caspase- 9 can also cleave the nuclear protein PARP. Northern blot analysis suggests that high expression of caspase-9 is found in the heart, testis and ovary.
- Format Purified
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.