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Permeabilizing Solution 2 RUO (GMP)

Permeabilizing Solution 2 

  • Brand BD FACS™
  • Application Flow cytometry (Routinely Tested) 
  • Storage Buffer Buffered solution with <15% formaldehyde, <50% diethylene glycol, and a proprietary permeabilizing agent.
  • Regulatory Status RUO (GMP)
Product Details Recommended Assay References

Description

BD FACS™ Permeabilizing Solution 2 (10X) is a premixed concentrate formulated specifically for use in flow cytometry. It is intended for the permeabilization of lymphocyte membranes prior to intracellular immunofluorescence staining with monoclonal antibodies. This formulation is designed for antigen-specific activation applications.

Resources & Tools
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Preparation and Storage

FACS Permeabilizing Solution 2 (10X) is stable for the period shown on the bottle label when stored as directed. Do not use this reagent if discoloration occurs or a precipitate forms. Store 1X solution at room temperature for up to 1 month.


  1. Asanuma H, Sharp M, Maecker HT, Maino VC, Arvin AM. Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus determined by intracellular detection of cytokine expression. J Infec Dis. In press. 2000;181:859-866.

  2. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+ CD27- lymphocyte subpopulation. J Immunol. 1995;154:4294-4301

  3. Komanduri KV, Viswanathan MN, Wieder ED, et al. Restoration of cytomegalovirus-specific CD4+ T-lymphocyte responses after ganciclovir and highly active antiretroviral therapy in individuals infected with HIV-1. Nat Med. 1998;4:953-956.

  4. Lee PP, Yee C, Savage PA, et al. Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients. Nat Ned. 1999;5:677-685.

  5. Nomura LE, Walker JM, Maecker HT. Optimization of whole blood antigen-specific cytokine assays for CD4+ T cells. Cytometry. In press. 2000;40:60-68.

  6. Picker LJ, Singh MK, Zdraveski Z, et al. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry. Blood. 1995;86:1408-1419.

  7. Pitcher CJ, Quittner C, Peterson DM, et al. HIV-1–specific CD4+ T cells are detectable in most individuals with active HIV-1 infection, but decline with prolonged viral suppression. Nat Med. 1999;5:518-525.

  8. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J Immunol Methods. 1998;212:89-98.

  9. Waldrop SL, Davis KA, Maino VC, Picker LJ. Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis. J Immunol. 1998;161:5284-5295.

  10. Waldrop SL, Pitcher CJ, Peterson DM, Maino VC, Picker LJ. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest. 1997;99:1739-1750.

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