Mouse IgG2a PE

BD™ Mouse IgG2a PE

Name: Mouse IgG2a PE
Brand: BD™
SKU: 340756

Clone X39

(ASR)

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Product Details

Brand: BD™

Isotype: Mouse BALB/c IgG2a

Immunogen: KLH

Application: Flow cytometry

Concentration: 25 µg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340756 Rev. 1

Antibody Details

X39

The Mouse IgG_2a antibody, clone X39, is derived from the hybridization of mouse Sp2/0-Ag 14 myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The Mouse IgG_2a antibody reacts specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.

340756 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340756 Rev.1

Citations & References

Development References (2)

Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).

340756 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.