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Mouse IgG<sub>1</sub> PerCP-Cy&trade;5.5 ASR

Mouse IgG1 PerCP-Cy™5.5 

Clone  X40   (ASR)

  • Concentration 25 µg/mL
  • Vol. Per Test 20 µL
  • Isotype Mouse BALB/c IgG1, κ
  • Application Flow cytometry, Isotype control (Routinely Tested) 
  • Immunogen Keyhole limpet hemocyanin (KLH)
  • Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
  • Regulatory Status ASR
Product Details Recommended Assay References

Description

The Mouse IgG1 antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.

Format
  • Format PerCP-Cy™5.5
  • Excitation Source Blue 488 nm
  • Excitation Max 482 nm
  • Emission Max 678 nm

PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7.

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Resources & Tools
 Spectrum Viewer  Download MSDS  Download TDS
Preparation and Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.


  1. Protection of Laboratory Workers from Occupationally Acquired Infections–Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.

  2. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.

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