Mouse IgG1 APC-Cy™7
Clone X40 (ASR)
- Concentration 100 µg/mL
- Vol. Per Test 5 µL
- Isotype Mouse BALB/c IgG1, κ
Flow cytometry, Isotype control (Routinely Tested)
- Immunogen Keyhole limpet hemocyanin (KLH)
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status ASR
Regulatory Status Legend
The Mouse IgG1 antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.
The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.
APC-Cy™7 is a tandem fluorochrome that combines APC and a cyanine dye (Cy7). Special precautions must be taken with APC-Cy7 conjugates, and cells stained with them, to protect the fluorochrome from long-term exposure to light. Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage. Due to nearly identical excitation and emission properties but different spillover characteristics, APC-Cy7 and APC-H7 cannot be used simultaneously.
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.