Clone S5.2 (ASR)
- Concentration 6.25 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse IgG2a
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Alloantigen-activated T Cells
- Workshop No. II T16
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status ASR
Regulatory Status Legend
The CD2 antibody, clone S5.2, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with T lymphocytes activated by mixed lymphocyte culture.
The CD2 antibody recognizes a human lymphocyte antigen, 45 to 50 kilodaltons (kDa), which also forms the binding site for sheep erythrocytes.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Preparation and Storage
Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.