BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
Uncover the whole transcriptome of a single cell
The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification kit enables unbiased 3'-based capture, amplification and detection of whole transcriptome at a single cell level. The BD Rhapsody WTA Amplification kit has been tested and validated to generate consistent, high-quality whole transcriptome data across different workflows, users and a wide range of cell inputs. With the BD® Single Cell Multiplexing kit (SMK) and the BD Rhapsody WTA Amplification kit working seamlessly together, you can save time and money by running multiple samples at once, giving you the option of multiplexing without compromising the quality of your data. The BD Rhapsody WTA Amplification kit brings whole transcriptome analyses of a single cell to your fingertips.
Trust your WTA data
Generating reproducible results across multiple users is critical for generating reliable, high-quality scientific data. The BD Rhapsody WTA Amplification kit generates consistent data across multiple users and between individual runs from a single user.
Generate consistent results across different cell inputs
The ability of an assay to generate consistent results across different cell inputs or different sample types that have varying cell numbers is critical. The BD Rhapsody WTA Amplification kit is designed work reliably across a wide range of cell inputs from 1,000 cells to 10,000 cells.
Process different samples together in a single WTA experiment
Processing multiple samples together in a single experiment also known as multiplexing is a cost effective and saves precious research time. The BD Rhapsody WTA Amplification kit works with the BD® Human Single-Cell Multiplexing kit to enable easy, reliable multiplexing of different samples.
Build targeted panels based on your WTA data
The WTA assay allows you to identify the most differentially expressed genes in your samples which can be then used to construct a targeted panel, specific for your research needs. Using the genes identified with the BD Rhapsody WTA Amplification kit create a fully custom panel with up to 500 targets or add up to 100 additional targets to one of already existing BD’s pre-designed panels.
Protocols and handbooks
- BD® Single-Cell Genomics Analysis Setup User Guide
- Single Cell Analysis Workflow with BD Rhapsody™ Systems
- BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
- BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol
- BD® Single-Cell Genomics Bioinformatics Handbook
- BD Rhapsody™ WTA Amplification Kit Product Insert
Multiomics protocols (BD WTA + BD AbSeq)
For Research Use Only. Not for use in diagnostic or therapeutic procedures.