Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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Depolarization of the plasma membrane during neurotransmitter release or muscle excitation/contraction involves increases in intracellular Ca2+. Homeostasis is reestablished through the action of various enzymes, including ion pumps. PMCAs (Plasma Membrane Calcium ATPases) belong to the P-type family of transport ATPases which couple ATP hydrolysis to the export of intracellular Ca2+. They are characterized by large molecular mass, high affinity for Ca2+, and direct regulation by interaction with Ca2+/calmodulin. Four different genes (PMCA 1-4) and independent alternative splice sites combine to generate multiple PMCA isoforms. PMCA 1-4 are highly homologous, but differ substantially in their N-terminal regions. PMCA1 and 4 are ubiquitously expressed and perform housekeeping roles. However, PMCA2 and 3 exhibit tissue-specific expression and perform more specialized functions. Although PMCA2 and 3 are both expressed to some degree in muscle, PMCA2 is primarily found in the cerebellum. There, it accounts for the majority of neural pump protein. Thus, PMCA2 is thought to be the principal ATPase that functions to maintain Ca2+ homeostasis in response to neural excitation.
Development References (4)
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Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997; 272(38):23741-23747. (Biology). View Reference
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Hilfiker H, Guerini D, Carafoli E. Cloning and expression of isoform 2 of the human plasma membrane Ca2+ ATPase. Functional properties of the enzyme and its splicing products. J Biol Chem. 1994; 269(42):26178-26183. (Biology). View Reference
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Shull GE, Greeb J. Molecular cloning of two isoforms of the plasma membrane Ca2+-transporting ATPase from rat brain. Structural and functional domains exhibit similarity to Na+,K+- and other cation transport ATPases. J Biol Chem. 1988; 263(18):8646-8657. (Biology). View Reference
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Stauffer TP, Hilfiker H, Carafoli E, Strehler EE. Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes. J Biol Chem. 1993; 268(34):25993-26003. (Biology). View Reference
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