PE Mouse Anti-Human CD1a

BD Pharmingen™ PE Mouse Anti-Human CD1a

Name: PE Mouse Anti-Human CD1a
Brand: BD Pharmingen™
SKU: 561754

Clone HI149

(RUO)

Profile of MOLT-4 cells analyzed on a FACScan (BDIS, San Jose, CA)

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Scientific Resources

Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: V 5T CD01.01

Entrez Gene ID: 909

RRID: AB_396141

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

For immunohistochemistry applications, Cat. No. 550366 is recommended.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

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Antibody Details

HI149

The HI149 monoclonal antibody specifically binds to CD1a. CD1a is a type I transmembrane glycoprotein. The 49 kDa CD1a polypeptide is associated with β2-microglobulin. CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation.

561754 Rev. 6

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

561754 Rev.6

Citations & References

Development References (4)

Calabi F, Bradbury A. The CD1 system. Tissue Antigens. 1991; 37(1):1-9. (Biology). View Reference
Hanau D, Schmitt DA, Bieber T, Schmitt D, Cazenave JP. Possible mechanism of action of CD1a antigens. J Invest Dermatol. 1990; 95(5):503-505. (Biology). View Reference
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

View All (4)

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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.