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      Biotin Mouse Anti-Canine CD34
      Product Details
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      BD Pharmingen™
      gp 105-120
      Mouse BALB/c IgG1, κ
      CD34-mouse IgG2a fusion protein and canine myelomonocytic leukemia ML3
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_2074492
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      550427 Rev. 7
      Antibody Details
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      2E9

      The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3% of cells are stained with 2E9 mAb; whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.

      550427 Rev. 7
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      550427 Rev.7
      Citations & References
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      Development References (10)

      1. Baumheter S, Singer MS, Henzel W, et al. Binding of L-selectin to the vascular sialomucin CD34. Science. 1993; 262(5132):436-438. (Biology). View Reference
      2. Bhattacharya V, McSweeney PA, Shi Q, et al. Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells. Blood. 2000; 95(2):581-585. (Biology). View Reference
      3. Bruno B, Nash RA, Wallace PM, et al. CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation. Transplantation. 1999; 68(3):338-344. (Clone-specific). View Reference
      4. Georges GE, Storb R, Bruno B, et al. Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes. Blood. 2001; 98(12):3447-3455. (Biology). View Reference
      5. Goerner M, Horn PA, Peterson L, et al. Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors. Blood. 2001; 98(7):2065-2070. (Clone-specific). View Reference
      6. Hagglund HG, McSweeney PA, Mathioudakis G, et al. Ex vivo expansion of canine dendritic cells from CD34+ bone marrow progenitor cells. Transplantation. 2000; 70(10):1437-1442. (Clone-specific). View Reference
      7. McSweeney PA, Rouleau KA, Storb R, et al. Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein. Blood. 1996; 88(6):1992-2003. (Biology). View Reference
      8. McSweeney PA, Rouleau KA, Wallace PM, et al. Characterization of monoclonal antibodies that recognize canine CD34. Blood. 1998; 91(6):1977-1986. (Immunogen). View Reference
      9. Niemeyer GP, Hudson J, Bridgman R, Spano J, Nash RA, Lothrop CD. Isolation and characterization of canine hematopoietic progenitor cells. Exp Hematol. 2001; 29(6):686-693. (Biology). View Reference
      10. Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction. Vet Immunol Immunopathol. 1999; 69(2-4):145-164. (Biology). View Reference
      View All (10) View Less
      550427 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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