PE Streptavidin

BD™ PE Streptavidin

Name: PE Streptavidin
Brand: BD™
SKU: 349023

(RUO (GMP))

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Application: Flow cytometry

Concentration: 5 µg/mL

Vol. Per Test: 20 μL

Storage Buffer: Buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

349023 Rev. 1

Antibody Details

Streptavidin is prepared chromatographically from Streptomyces avidinii. When excited by a laser beam at 488 nm, Streptavidin PE emits red-orange light with an emission maximum at approximately 580 nm. The emitted light is collected in the fluorescence 2 (FL2) channel.

349023 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

349023 Rev.1

Citations & References

Development References (4)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Green NM. Avidin. Adv Protein Chem. 1975; 29:85-133. (Biology).
Parks DR, Lanier LL, Herzenberg LA. Weir DM, ed. Handbook of Experimental Immunology. Oxford: Blackwell Scientific Publications; 1986:29.

View All (4)

349023 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.