T 세포 면역학

BD는 다양한 테크놀로지를 이용하여, T cell을 다른 관점에서 연구하기 위한 종합적인 시약과 키트의 포트폴리오를 제공합니다.

T cell을 연구하는 방법 중에서, multicolor flow cytometry는 매우 복잡한 T cell subpolulation의 기능적, 표현형적 특징을 분석할 수 있게 하는 탁월한 실험 방법입니다. ELISA, ELISPOT, bead-based immunoassay와 같은 보완적인 기술 또한 다양한 실험적인 요구에 부합하는 유연성과 결과를 검증하는 다양한 방법을 제공하여 T cell 분야에서 더 깊은 연구를 할 수 있게 합니다. 

Comparison of technologies for T cell research

The following table lists important characteristics of tools to help you find the ones that meet your particular experimental needs. Certain technologies can reveal specific information about a sample, or might better meet practical needs such as the available instrumentation or sample type. In some cases, researchers can use the combined information from multiple techniques to verify results. Different approaches can paint a detailed picture of the mechanisms contributing to T-cell development.

Tool/Technology Flow Cytometry: Surface Flow Cytometry: Intracellular BD™ Cytometric Bead Array (CBA) ELISPOT ELISA In Vivo Capture Assay
Molecules detected Surface Intracellular and surface Secreted or intracellular Secreted (in situ) Secreted Secreted (in vivo)
Multiparameter Yes Yes Yes No No No
Single cell/cell subset
Yes Yes No Frequencies, no subset
No No
Antigen-specific Yes Yes Yes Yes Yes Yes
Post-assay viability Yes No Yes, for secreted molecules No Yes Yes
Quantitation of protein Possible* Possible* Yes No Yes Yes
Instrumentation Flow cytometer Flow cytometer Flow cytometer ELISPOT reader Spectrophotometer Spectrophotometer

*With a standard such as BD Quantibrite™ beads

Immunophenotyping: combining surface and intracellular

Using multicolor flow cytometry—and combining surface phenotyping with intracellular staining of cytokines, transcription factors, or signaling molecules—you can identify individual T-cell subsets on the basis of their characteristic marker signature. BD's advanced reagent systems, extensive marker selection, and wealth of support tools help researchers simplify phenotyping and maximize the information obtained from individual samples.

Intracellular cytokines

BD Cytofix/Cytoperm™ reagents and the BD FastImmune™ cytokine system are reagents and protocols optimized to help you use flow cytometry to reproducibly measure cytokines within individual cells.

Intracellular signaling

BD Phosflow™ reagents help you use multicolor flow cytometry to reliably measure the level of phosphorylated proteins involved in T-cell signaling, and combine the data with subset identification.

Secreted cytokines

For detection of secreted cytokines within a sample, BD offers multiple assays. BD Cytometric Bead Array (CBA) technology allows quantitation of multiple soluble cytokines simultaneously, while BD OptEIA™ ELISA reagents are designed for quantitation of single cytokines. BD™ ELISPOT reagents enable determination of the frequency of cytokine-producing cells, and BD™ In Vivo Capture Assays allow quantitation by directly capturing cytokines in vivo.