BD Cytofix/Cytoperm Method

Intracellular cytokine staining with flexibility of experimental design and the confidence of established reagents

The BD Cytofix/Cytoperm™ method and related reagents address the wide-ranging experimental needs of scientists conducting basic research. With reagents for a broad selection of cytokines and multiple species, this method offers maximum flexibility in intracellular cytokine staining.


The BD Cytofix/Cytoperm method and related reagents have been developed with the need for flexibility in experimental design in mind. The protocol has been developed to work not only in human cells, but also in non-human primate, mouse, and rat samples. The method is compatible with a wide range of samples and offers more cytokines and multicolor options.

Product List

Product List

BD Biosciences offers cell fixation and permeabilization kits to simplify the preparation of cells for intracellular staining of cytokines. Not only are they convenient, they also add an additional factor of standardization to intracellular staining assays: using quality control tested fixation reagents assures consistent lot-to-lot results.