BD LSRFortessa X-20

Greater Consistency

BD FACSDiva™ software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa™ X-20 workstation. The software is common across BD FACS™ instrument platforms, including the BD FACSCanto™ cell analyzer and BD FACSAria™ cell sorter systems. This affords researchers greater application flexibility, allowing them to easily move assays from one platform to another.

Cytometer Setup and Tracking

The BD™ Cytometer Setup and Tracking (CS&T) feature of BD FACSDiva software establishes baseline settings and adjusts for instrument variability. The software reduces operator error, and ensures consistency of results by setting the signal time delay across the multiple laser beams and optimizing PMT voltages. Application-specific settings can also be specified, allowing for rapid setup and performance of routine experiments in a more consistent manner. QC tracking capabilities in the software measure instrument settings and report on performance. Levey-Jennings plots help users understand instrument performance and identify maintenance issues.

BD LSRFortessa X-20 front panel
BD LSRFortessa X-20 front panel
Analysis of human T-cell subsets
Analysis of human T-cell subsets
Data shows a 10-color panel run on a 3-laser BD LSRFortessa X-20 with a blue/red/violet configuration. The panel was used for defining various subsets of regulatory T cells (A, B), memory/effector CD4+ T cells (C, D, E, F), and memory/effector CD8+ T cells (G, H, I, J).


Acquisition and Analysis

BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. Acquisition templates, user-defined experiment layouts, and simple compensation procedures are also managed by the software to further facilitate acquisition.

For analysis, the software includes powerful features such as hierarchical snap-to gating, a variety of plot formats, and batch analysis. Recorded data can be analyzed by creating plots, gates, population hierarchies, and statistics views on a BD FACSDiva global worksheet. Once the global worksheet is saved, it can be used to analyze multiple sample tubes from an experiment, thereby saving time.

Numerous other productivity benefits include user-defined batch analysis and automated features such as gate resizing, pausing between data files, exporting statistics, and printing before proceeding to the next data file.