BD LSRFortessa

Maximum Signal, Minimum Crosstalk

The patented collection optics are yet another design innovation. Arranged in octagon- and trigon-shaped optical pathways, their novel design efficiently maximizes signal detection and increases sensitivity and resolution. This allows researchers to identify cells, especially dim and rare cell populations, optimizing multicolor assays and panel design for superior results.

Optics System

The optics system consists of laser excitation optics that illuminate cells in the sample, and collection optics that direct light scatter and fluorescence signals through spectral filters to detectors. Innovative designs for both the excitation optics and collection optics in BD LSRFortessa systems reduce excitation losses and optimize collection efficiency for increased sensitivity and resolution.

The BD LSRFortessa is designed to enable your current assay requirements. As future needs arise, lasers can be added or upgraded.

Excitation Optics

The excitation optics consist of multiple fixed wavelength lasers, beam shaping optics, and individual pinholes which result in spatially separated beam spots.

A final lens focuses the laser light into the gel-coupled cuvette flow cell. Since the optical pathway and the sample core stream are fixed, alignment is constant from day to day and from experiment to experiment.

Collection Optics

Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The collection optics are set up in patented octagon- and trigon-shaped optical pathways that maximize signal detection resulting from each laser illuminated beam spot. Bandpass filters in front of each PMT allow spectral selection of the collected wavelengths. Importantly, this arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.

Optics - Transmission Pathways Parsley 1

Transmission pathways in an octagon

Optics - Emission Spectra Parsley 2

Emission spectra of commonly used fluorochromes

This design is based on the principle that light reflection is more efficient than light transmission. Emitted light travels to each PMT detector via reflection and is transmitted through only two pieces of glass to reach the detector. Therefore, more colors can be detected with minimum light loss.