BD FACSCanto Research System flow cytometer

High performance, innovative system

The BD FACSCanto fluidics system is designed to streamline work, save time, and improve performance.

In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate.

Fluidics cart

A fluidics cart holds large fluid tanks necessary to operate and maintain the instrument. For sample acquisition, positivepressure pumps in the fluidics cart send sheath fluid past a 0.2-μm filter to a pressurized interior reservoir inside the instrument called the plenum. The plenum maintains a nearly constant fluid level and dampens pump pulsation using a new dynamic feedback pressure control system designed to regulate pressure. As a result, sheath flow rate does not vary with the level of fluid in the sheath cubitainer, and the reservoir automatically removes small air bubbles from the sheath supply.

Features Fluidics - Flow Cell Image

Flow cell

Startup, shutdown, and cleaning procedures

Daily routine procedures, such as startup, shutdown, and cleaning routine cycles, are automated. BD FACS™ Shutdown solution prevents salt crystal buildup in fluidics lines and is supplemented with a preservative to prevent bacterial growth. During the instrument shutdown procedure, BD FACS Shutdown solution replaces sheath fluid in all sample and sheath fluid lines.

Features Fluidics - Hydronamic Focusing Image

Hydrodynamic focusing

A high flow rate is generally used for measurements such as immunophenotyping, for which data is less resolved but is acquired more quickly. A lower flow rate is generally used in applications for which optimal resolution and sensitivity are critical.

Example of a 10-color panel acquired on a BD FACSCanto system

Example of a 10-color panel acquired on a BD FACSCanto system

The panel was used for identifying various lymphocyte subsets: T-cells (represented in blue) and non-T-cell subsets (represented in orange: NK and B cells). Staining was done using lysed whole blood (BD FACS™ lysing solution) from a normal donor.