제품 세부 정보
BD Pharmingen™
Mouse (QC Testing)
null IgG1
Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
0.5 mg/ml
AB_10167545
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
준비 및 보관
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Avoid multiple freeze-thaws of product.
The fusion protein solution should be stored at -20˚C until the vial is opened. After opening, undiluted aliquots should be stored at -20˚C or below. Thawed aliquots may be stored up to two months at 4˚C.
권장 분석 절차
For detection of the fusion protein, we recommend a polyclonal anti-hman Ig second step. Other reported applications include affinity isolation of P-selectin ligand.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
Lysing Buffer RUO
사이즈
100 ML
카탈로그 No
555899
Cell Viability Solution RUO
사이즈
500 Tests
카탈로그 No
555815
555294 Rev. 8
항체 세부 정보
p-Selectin-Ig CHO
The Mouse P-selectin - IgG Fusion Protein is composed of the signal sequence, the lectin domain, the EGF-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2 and CH3) of human IgG1. This chimeric protein binds to P-selectin ligand expressed on mouse neutrophils and myeloid cell lines. Furthermore, P-selectin-IgG fusion protein selectively stains Th1 cells, but not Th2 cells.reported in the literature.
555294 Rev. 8
형광 세부 정보
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555294 Rev.8
인용 및 참고 문헌
View product citations for antibody "555294" on CiteAb
개발 참고 자료 (4)
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Austrup F, Vestweber D, Borges E, et al. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues. Nature. 1997; 385(6611):81-83. (Biology). 참조 보기
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Hahne M, Jager U, Isenmann S, Hallmann R, Vestweber D. Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes.. J Cell Biol. 1993; 121(3):655-664. (Biology). 참조 보기
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Katakai T, Mori KJ, Masuda T, Shimizu A. Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis. Int Immunol. 2002; 14(2):167-175. (Biology). 참조 보기
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Lenter M, Levinovitz A, Isenmann S, Vestweber D. Monospecific and common glycoprotein ligands for E- and P-selectin on myeloid cells. J Cell Biol. 1994; 125(2):471-481. (Biology). 참조 보기
555294 Rev. 8
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.