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PE Mouse Anti-c-Maf
제품 세부 정보
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BD Pharmingen™
MAF; v-MAF; MAF; AYGRP; CCA4; CTRCT21
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG2a, κ
Human c-MAF Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739359
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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제품 고시

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. An isotype control should be used at the same concentration as the antibody of interest.
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565795 Rev. 1
항체 세부 정보
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T54-853

The T54-853 monoclonal antibody recognizes c-Maf, a transcription factor that is also known as, MAF, c-Maf protooncogene, or V-maf musculoaponeurotic fibrosarcoma oncogene homolog. c-Maf belongs to the basic leucine zipper (bZIP) family of transcription factors. c-Maf acts as a homodimer or can dimerize with other bZIP transcription factors to either activate or repress the expression of various genes. It plays multiple roles in the differentiation and effector functions of leucocytes, including macrophages, Th2 cells, Th17 cells, and T follicular helper (Tfh) cells. c-Maf drives the expression of IL-10 by activated macrophages, IL-21 by activated Tfh and Th17 cells, and IL-4 by Th2 cells.

565795 Rev. 1
형광 세부 정보
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
496 nm, 566 nm
576 nm
565795 Rev.1
인용 및 참고 문헌
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View product citations for antibody "565795" on CiteAb

개발 참고 자료 (8)

  1. Apetoh L, Quintana FJ, Pot C, et al. The aryl hydrocarbon receptor interacts with c-Maf to promote the differentiation of type 1 regulatory T cells induced by IL-27.. Nat Immunol. 2010; 11(9):854-61. (Biology). 참조 보기
  2. Barros MH, Hauck F, Dreyer JH, Kempkes B, Niedobitek G. Macrophage polarisation: an immunohistochemical approach for identifying M1 and M2 macrophages.. PLoS ONE. 2013; 8(11):e80908. (Biology). 참조 보기
  3. Dhiman R, Bandaru A, Barnes PF, et al. c-Maf-dependent growth of Mycobacterium tuberculosis in a CD14(hi) subpopulation of monocyte-derived macrophages.. J Immunol. 2011; 186(3):1638-45. (Biology). 참조 보기
  4. Grigoryan G, Reinke AW, Keating AE. Design of protein-interaction specificity gives selective bZIP-binding peptides.. Nature. 2009; 458(7240):859-64. (Biology). 참조 보기
  5. Hiramatsu Y, Suto A, Kashiwakuma D, et al. c-Maf activates the promoter and enhancer of the IL-21 gene, and TGF-beta inhibits c-Maf-induced IL-21 production in CD4+ T cells.. J Leukoc Biol. 2010; 87(4):703-12. (Biology). 참조 보기
  6. Ho IC, Hodge MR, Rooney JW, Glimcher LH. The proto-oncogene c-maf is responsible for tissue-specific expression of interleukin-4.. Cell. 1996; 85(7):973-83. (Biology). 참조 보기
  7. Kroenke MA, Eto D, Locci M, et al. Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation.. J Immunol. 2012; 188(8):3734-44. (Biology). 참조 보기
  8. van den Bosch MW, Palsson-Mcdermott E, Johnson DS, O'Neill LA. LPS induces the degradation of programmed cell death protein 4 (PDCD4) to release Twist2, activating c-Maf transcription to promote interleukin-10 production.. J Biol Chem. 2014; 289(33):22980-90. (Biology). 참조 보기
모두 보기 (8) 간단히 보기
565795 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.