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APC-R700 Mouse Anti-Human CD3 SK7 RUO

APC-R700 Mouse Anti-Human CD3 

Clone  SK7   (RUO)

  • Brand BD Horizon™
  • Concentration 25 µg/mL
  • Vol. Per Test 5 µL
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Human Thymocytes
  • Workshop No. II T118; III T492
  • Entrez Gene ID 916 
  • Storage Buffer Phosphate buffered saline (PBS) containing BSA and ProClin.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The CD3 antibody, clone SK7,15,16 is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human thymocytes.

The CD3 antibody reacts with the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

Format
  • Format APC-R700
  • Excitation Source Red 633 nm
  • Excitation Max 652 nm
  • Emission Max 704 nm

APC-R700 is a tandem fluorochrome that combines APC with R700, a proprietary organic dye. This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. Due to similar excitation and emission properties, APC-R700 and Alexa Fluor® 700 cannot be used simultaneously.

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Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Regulatory Document Website
Preparation and Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.


  1. Allison JP, Lanier LL. Structure, function, and serology of the T-cell antigen receptor complex. Ann Rev Immunol. 1987;5:503-540.

  2. Brenner MB, Groh V, Porcelli SA, et al. Structure and distribution of the human / T-cell receptor. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1049-1053.

  3. Campbell MJ, Scott J, Maecker HT, Park JW, Esserman LJ. Immune dysfunction and micrometastases in women with breast cancer. Breast Cancer Res Treat. 2005;91:163-171.

  4. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.

  5. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annu Rev Immunol. 1988;6:629-662.

  6. Friedrich W, O'Reilly RJ, Kozinger B, Gebhard DF, Good RA, Evans RL. T lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: imbalances of T-cell subpopulations having unique regulatory and cognitive functions. Blood. 1982;59:696-701.

  7. Garson JA, Beverley PCL, Coakham HB, Harper EI. Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982;298:375-377.

  8. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. Vol 1. New York, NY: Springer-Verlag; 1986:3-30.

  9. Kaneoka H, Perez-Rojas G, Sasasuki T, Benike CJ, Engleman EG. Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu-4): heterogeneity of response is a function of monocytes. J Immunol. 1983;131:158-164.

  10. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. Vol 1. New York, NY: Springer-Verlag; 1986:259-288.

  11. Kurrle R. Cluster report: CD3. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:290-293.

  12. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986;137:2501-2507.

  13. Maecker HT, Maino VC. Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometry. Hum Immunol. 2004;65:493-499.

  14. Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression. Cytometry. 1998;34:207-215.

  15. Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline— Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.

  16. Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991;60:190-208.

  17. Tu W, Chen S, Sharp M, et al. Persistent and selective deficiency of CD4+T cell immunity to cytomegalovirus in immunocompetent young children. J Immunol. 2004;172:3260-3267.

  18. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.

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