V450 Mouse Anti-Human Light Chain, λ

BD Horizon™ V450 Mouse Anti-Human Light Chain, λ

Name: V450 Mouse Anti-Human Light Chain, λ
Brand: BD Horizon™
SKU: 561379

クローン JDC-12

(RUO)

Flow cytometric analysis of Ig, λ light chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC-H7 Anti-Human CD19 antibody (Cat. No. 560727) and with either BD Horizon™ V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373; dashed line histogram) or BD Horizon™ V450 Mouse anti-Human Ig, λ light chain antibody (Cat. No. 561379; solid line histogram). The fluorescence histograms were derived from CD19+ gated events with the forward- and side-light scattering characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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製品詳細

Brand: BD Horizon™

Alternative Name: Ig λ; IGL@; IGL; Immunoglobulin lambda locus

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_10681718

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.

推奨アッセイ手順

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

V450 Mouse IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 560373

APC-H7 Mouse Anti-Human CD19 RUO

サイズ 50 Tests カタログ番号 560727

561379 Rev. 2

抗体の詳細

JDC-12

The JDC-12 monoclonal antibody specifically binds to human immunoglobulin light chain, lambda (λ). It does not bind to immunoglobulin κ light chains or heavy chains.

The antibody is conjugated to BD Horizon V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon V450 can be used in place of Pacific Blue™ conjugates.

561379 Rev. 2

フォーマットの詳細

V450

BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: V450

励起源: Violet 405 nm

最大励起波長: 405 nm

最大蛍光波長: 450 nm

561379 Rev.2

引用＆参考文献

Development References (2)

Odendahl M, Jacobi A, Hansen A, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000; 165(10):5970-5979. (Clone-specific: Flow cytometry). View Reference
Sembries S, Pahl H, Stilgenbauer S, Döhner H, Schriever F. Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.. Blood. 1999; 93(2):624-31. (Clone-specific: Flow cytometry). View Reference

561379 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.