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Biotin Rat Anti-Mouse IgG2a R19-15 RUO

Biotin Rat Anti-Mouse IgG2a 

Clone  R19-15   (RUO)

  • Brand BD Pharmingen™
  • Concentration 250 µg/ml
  • Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
  • Reactivity Mouse (QC Testing) 
  • Application ELISA (Routinely Tested) 
    Immunohistochemistry-formalin (antigen retrieval required), Flow cytometry, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development) 
  • Immunogen Pooled BALB/c and C57BL/6 mouse Ig
  • Entrez Gene ID 380793 
  • Storage Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

Format
  • Format Biotin

Other Formats →

Suggested Companion Products

DAB Substrate Kit RUO
500 Tests Cat No: 550880

Streptavidin HRP RUO
50 mL Cat No: 550946

Antibody Diluent for IHC RUO
125 mL Cat No: 559148

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


This antibody conjugate has been tested as a detecting reagent in ELISA (~2 µg/ml) to assure purity and reactivity.

For immunohistochemical staining on acetone-fixed frozen sections, a dilution range of 1:25 - 1:100 is suggested.


  1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. View reference

  2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. View reference

550332 Rev. 5
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