Biotin Rat Anti-Mouse IgG2a
Clone R19-15 (RUO)
- Brand BD Pharmingen™
- Concentration 250 µg/ml
- Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
- Reactivity Mouse (QC Testing)
ELISA (Routinely Tested)
Immunohistochemistry-formalin (antigen retrieval required), Flow cytometry, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
- Immunogen Pooled BALB/c and C57BL/6 mouse Ig
- Entrez Gene ID 380793
- Storage Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
Suggested Companion Products
|Resources & Tools|
|Spectrum Viewer||Panel Designer||Spectrum Viewer||Download TDS||Regulatory Document Website|
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
This antibody conjugate has been tested as a detecting reagent in ELISA (~2 µg/ml) to assure purity and reactivity.
For immunohistochemical staining on acetone-fixed frozen sections, a dilution range of 1:25 - 1:100 is suggested.