Purified Mouse Anti-Pig Monocyte/Granulocyte
Clone 74-22-15A (RUO)
- Brand BD Pharmingen™
- Alternative Name SWC3; CD172a
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2b, κ
- Reactivity Pig (QC Testing)
Flow cytometry (Routinely Tested)
Immunoprecipitation, Depletion, Immunohistochemistry-frozen (Reported)
- Immunogen dd miniature swine thymocytes
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 74-22-15A (switch variant of 74-22-15) monoclonal antibody, an isotype class-switch variant of mAb 74-22-15, specifically binds to a 230-kDa protein expressed by most pig macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes. mAb 74-22-15A does not crossreact with human or bovine cells. This clone was clustered as anti-SWC3a at the First International Swine CD workshop.
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Other reported applications include immunoprecipitation, complement-mediated depletion, and immunohistochemical staining of frozen sections.