FITC Mouse Anti-Pig Monocyte/Granulocyte
Clone 74-22-15A (RUO)
- Brand BD Pharmingen™
- Alternative Name SWC3; CD172a
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2b, κ
- Reactivity Pig (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen dd miniature swine thymocytes
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 74-22-15A (switch variant of 74-22-15) monoclonal antibody, an isotype class-switch variant of mAb 74-22-15, specifically binds to a 230-kDa protein expressed by most pig macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes. mAb 74-22-15A does not crossreact with human or bovine cells. This clone was clustered as anti-SWC3a at the First International Swine CD workshop.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.