FITC Mouse Anti-Rat RT1D
Clone OX-17 (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Rat (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Wistar rat splenocyte membrane extract
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The OX-17 antibody reacts with a non-polymorphic determinant of rat MHC Class II antigen, I-E equivalent. RT1D is found on peripheral B lymphocytes, thymic cortical epithelial and medullary reticular cells, epithelial cells in the adult small intestine, macrophages and dendritic cells in the intestinal lamina propria and Peyer's patches, epithelial and dendritic cells in the kidney, and activated monocytes, but not on thymocyes, erythrocytes, peripheral T cells, or brain. Its expression is upregulated on large-vessel endothelia and a variety of epithelial and glandular cells in the gastrointestinal system of rats treated with IFN-γ.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.