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FITC Rat anti-Mouse CD172a
FITC Rat anti-Mouse CD172a
Flow cytometric analysis of FITC-conjugated anti-mouse CD172a on mouse bone marrow. Bone marrow from BALB/c mice were stained with FITC Rat anti-Mouse CD172a and APC Rat Anti-Mouse CD11b (clone M1/70, Cat. No. 553312) and analyzed by flow cytometry.  Flow cytometry was performed on a BD FACSCalibur™ System and the contour plot was derived from the gated events based on light scattering characteristics of viable bone marrow cells.
Flow cytometric analysis of FITC-conjugated anti-mouse CD172a on mouse bone marrow. Bone marrow from BALB/c mice were stained with FITC Rat anti-Mouse CD172a and APC Rat Anti-Mouse CD11b (clone M1/70, Cat. No. 553312) and analyzed by flow cytometry.  Flow cytometry was performed on a BD FACSCalibur™ System and the contour plot was derived from the gated events based on light scattering characteristics of viable bone marrow cells.
製品詳細
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BD Pharmingen™
Sirpa; Sirp alpha; Sirpα; Bit; SHPS-1; p84; Ptpns1
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Membrane fractions from mouse brain
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_1645240
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. An isotype control should be used at the same concentration as the antibody of interest.
560316 Rev. 2
抗体の詳細
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P84

The P84 monoclonal antibody specifically binds to CD172a, also known as SIgnal-Regulatory Protein α (SIRPα), Src Homology 2 domain-containing protein tyrosine Phosphatase (SHP) Substrate 1 (SHPS-1), or Brain Immunoglobulin-like molecule with Tyrosine-based activation motifs (BIT). CD172a is an adhesion molecule of the Ig superfamily which is expressed on neurons in the central nervous system and the retina, on macrophages, and on bone-marrow myeloid cells. Its ligand, CD47, or Integrin-Associated Protein (IAP), is expressed by a wide variety of cells. CD172a and CD47 are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages.

560316 Rev. 2
フォーマットの詳細
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
560316 Rev.2
引用&参考文献
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Development References (7)

  1. Chuang W, Lagenaur CF. Central nervous system antigen P84 can serve as a substrate for neurite outgrowth. Dev Biol. 1990; 137(2):219-232. (Immunogen). View Reference
  2. Comu S, Weng W, Olinsky S. The murine P84 neural adhesion molecule is SHPS-1, a member of the phosphatase-binding protein family. J Neurosci. 1997; 17(22):8702-8710. (Biology). View Reference
  3. Gresham HD, Dale BM, Potter JW, et al. Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr. J Exp Med. 2000; 191(3):515-528. (Biology). View Reference
  4. Jiang P, Lagenaur CF, Narayanan V. Integrin-associated protein is a ligand for the P84 neural adhesion molecule. J Biol Chem. 1999; 274(2):559-562. (Biology). View Reference
  5. Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina. J Comp Neurol. 2000; 416(3):335-344. (Biology). View Reference
  6. Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Role of CD47 as a marker of self on red blood cells. Science. 2000; 288(5473):2051-2054. (Biology). View Reference
  7. Veillette A, Thibaudeau E, Latour S. High expression of inhibitory receptor SHPS-1 and its association with protein-tyrosine phosphatase SHP-1 in macrophages. J Biol Chem. 1998; 273(35):22719-22728. (Biology). View Reference
すべて表示する (7) 表示項目を減らす
560316 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.