Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      FITC Mouse Anti-Mouse CD244.2
      FITC Mouse Anti-Mouse CD244.2
      Two-color analysis of CD244.2 expression on LAK cells. C57BL/6 LAK cells were simultaneously stained with PE Mouse anti-Mouse NK-1.1 (Cat. No. 557391/553165, both panels) and FITC Mouse anti-Mouse CD244.2(right panel) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).
      FITC Mouse Anti-Mouse CD244.2
      Two-color analysis of CD244.2 expression on LAK cells. C57BL/6 LAK cells were simultaneously stained with PE Mouse anti-Mouse NK-1.1 (Cat. No. 557391/553165, both panels) and FITC Mouse anti-Mouse CD244.2(right panel) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).
      製品詳細
      Down Arrow Up Arrow


      BD Pharmingen™
      CD242.2; 2B4 B6 Alloantigen; Ly90; NAIL; Nmrk; SLAMF4
      Mouse (QC Testing)
      Mouse 129, also known as 129/J or 129/SvJ IgG2b, κ
      rIL-2-propagated NK1.1+ cells from C57BL/6 mice
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      18106
      AB_394769
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      553305 Rev. 15
      抗体の詳細
      Down Arrow Up Arrow
      2B4

      CD244 is a type I transmembrane glycoprotein that is also known as Natural killer cell receptor 2B4 (NKR2B4), or Signaling lymphocytic activation molecule 4 (SLAMF4). CD244 is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF). It is expressed on all natural killer (NK) cells, IL-2-activated NK (LAK) cells, committed progenitors of NK cells, and a subset of T lymphocytes which mediate non-MHC-restricted cytoxicity, including dendritic epidermal T cells. The 2B4 monoclonal antibody specifically binds to CD244.2, the 2B4 alloantigen which is expressed on C57BL/6 and C58/J mice, but not in most strains tested (A/J, AKR, BALB/c, CBA/J, CBA/N, C3H/He, C57BR, DBA/1, DBA/2, NZB, SJL/J, 129). At least two isoforms of CD244.2 protein, products of alternative splicing of hnRNA, are expressed on IL-2-activated C57BL/6 NK cells. They differ only in their cytoplasmic domains; with 2B4L (150-aa cytoplasmic tail) having inhibitory activity and 2B4S (93-aa tail) being stimulatory. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. 2B4 antibody activates lytic and secretory functions of IL-2-cultured NK cells and CD244.2+ T cells.

      553305 Rev. 15
      フォーマットの詳細
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      553305 Rev.15
      引用＆参考文献
      Down Arrow Up Arrow

      Development References (13)

      1. Brown MH, Boles K, van der Merwe PA, Kumar V, Mathew PA, Barclay AN. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J Exp Med. 1998; 188(11):2083-2090. (Biology). View Reference
      2. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
      3. Garni-Wagner BA, Purohit A, Mathew PA, Bennett M, Kumar V. A novel function-associated molecule related to non-MHC-restricted cytotoxicity mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(1):60-70. (Biology). View Reference
      4. Kubota K, Katoh H, Muguruma K, Koyama K. Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen. Immunology. 1999; 96(3):491-497. (Biology). View Reference
      5. Kumaresan PR, Stepp SE, Bennett M, Kumar V, Mathew PA. Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4. Immunogenetics. 2000; 51(4-5):306-313. (Biology). View Reference
      6. Latchman Y, McKay PF, Reiser H. Identification of the 2B4 molecule as a counter-receptor for CD48. J Immunol. 1998; 161(11):5809-5812. (Biology). View Reference
      7. Mathew PA, Garni-Wagner BA, Land K, et al. Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(10):5328-5337. (Biology). View Reference
      8. Rosmaraki EE, Douagi I, Roth C, Colucci F, Cumano A, Di Santo JP. Identification of committed NK cell progenitors in adult murine bone marrow. Eur J Immunol. 2001; 31(6):1900-1909. (Biology). View Reference
      9. Schatzle JD, Sheu S, Stepp SE, Mathew PA, Bennett M, Kumar V. Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4. Proc Natl Acad Sci U S A. 1999; 96(7):3870-3875. (Biology). View Reference
      10. Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A. 2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors. J Invest Dermatol. 1995; 105(4):592-596. (Biology). View Reference
      11. Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A. Activation of murine epidermal gamma delta T cells through surface 2B4. Eur J Immunol. 1995; 25(4):1117-1120. (Biology). View Reference
      12. Sentman CL, Hackett J Jr, Moore TA, Tutt MM, Bennett M, Kumar V. Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen. Hybridoma. 1989; 8(6):605-614. (Immunogen). View Reference
      13. Stepp SE, Schatzle JD, Bennett M, Kumar V, Mathew PA. Gene structure of the murine NK cell receptor 2B4: presence of two alternatively spliced isoforms with distinct cytoplasmic domains. Eur J Immunol. 1999; 29(8):2392-2399. (Biology). View Reference
      すべて表示する (13) 表示項目を減らす
      553305 Rev. 15

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback