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FITC Hamster IgG2 κ Isotype Control
製品詳細
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BD Pharmingen™
Anti-KLH
Armenian Hamster IgG2, κ
Keyhole Limpet Hemocyanin
Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
0.5 mg/ml
AB_393545
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). This product is not recommended for use as an isotype control for intracellular or immunohistochemical staining applications.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550056 Rev. 2
抗体の詳細
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B81-3

The B81-3 monoclonal antibody was raised against keyhole limpet hemocyanin (KLH), an antigen not expressed by mammalian cells or cell lines. Intended for use as a hamster immunoglobulin isotype control, the B81-3 antibody can be used as a negative control to help differentiate non-specific background signal when compared against a signal from a specific hamster antibody. The purified B81-3 antibody may also serve as a useful standard for some ELISA applications. KLH is a oxygen carrying metalloprotein from a species of keyhole limpet that lives off the coast of California and is often used as a carrier protein to help elicit immune responses to haptens. Since KLH is phylogenetically distant from many mammalian proteins, it often has very minimal to no cross-reactivity for many immunological assays.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

550056 Rev. 2
フォーマットの詳細
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
550056 Rev.2
引用&参考文献
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Development References (1)

  1. Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen). View Reference
550056 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.