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FITC Mouse Anti-Human Vγ9 TCR
FITC Mouse Anti-Human Vγ9 TCR
Flow cytometric analysis of Vγ9 TCR expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Vγ9 TCR (Cat. No. 555732) and PE Mouse Anti-Human CD3 (Cat. No. 555333/561808/561809). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent two-color dot plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
Flow cytometric analysis of Vγ9 TCR expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Vγ9 TCR (Cat. No. 555732) and PE Mouse Anti-Human CD3 (Cat. No. 555333/561808/561809). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent two-color dot plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
製品詳細
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BD Pharmingen™
Vgamma9 TCR; V2; TRGC1; TCRGV9; TRGV9
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
6983
AB_396075
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555732 Rev. 7
抗体の詳細
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B3

The B3 monoclonal antibody specifically recognizes the human variable γ9 subunit (Vγ9) of γ/δ T-cell receptor (TCR). The γ/δ TCR is composed of two disulfide-linked glycoproteins. The gamma chain is approximately 45-60 kD and is detected on a small subset peripheral blood T cells and thymocytes. The majority of normal peripheral blood γ/δ T cells express a Vγ9+/Vδ2+ phenotype. The reason for this selection in the T-cell repertoire is not well understood.

555732 Rev. 7
フォーマットの詳細
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
555732 Rev.7
引用&参考文献
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Breit TM, Wolvers-Tettero IL, van Dongen JJ. Receptor diversity of human T-cell receptor gamma delta expressing cells. Prog Histochem Cytochem. 1992; 26(1-4):182-193. (Biology). View Reference
  3. Kabelitz D. Function and specificity of human gamma/delta-positive T cells. Crit Rev Immunol. 1992; 11(5):281-303. (Biology). View Reference
555732 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.