FITC Mouse Anti-Human Vγ9 TCR
Clone B3 (RUO)
- Brand BD Pharmingen™
- Alternative Name Vgamma9 TCR; V2; TRGC1; TCRGV9; TRGV9
- Concentration 0.5 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Entrez Gene ID 6983
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The B3 monoclonal antibody specifically recognizes the human variable ã9 subunit (Vγ9) of ã/δ T-cell receptor (TCR). The γ/δ TCR is composed of two disulfide-linked glycoproteins. The gamma chain is approximately 45-60 kD and is detected on a small subset peripheral blood T cells and thymocytes. The majority of normal peripheral blood γ/δ T cells express a Vγ9+/Vδ2+ phenotype. The reason for this selection in the T-cell repertoire is not well understood.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.