Description
The HP-3D9 monoclonal antibody specifically binds to CD94 which is also known as KP43. CD94 is expressed on the cell surface as a ~70 kDa, disulfide-linked, type II transmembrane glycoprotein dimer. It is encoded by KLRD1 (Killer cell lectin like receptor D1) which belongs to the C-type lectin superfamily. CD94 is expressed on natural killer (NK) cells, especially activated NK cells. It is also expressed on ã/ä TCR+ T lymphocytes, NK-T cells, and on some CD8+CD56+ α/β TCR+ cells. CD94 associates with various NKG2 receptors to form receptors for HLA class I molecules and plays a role in regulating cellular adhesion and activation. The HP-3D9 antibody can reportedly inhibit the cytolytic activity of activated NK cells.
Format
- Format
FITC
- Excitation Source
Blue 488 nm
- Excitation Max
494 nm
- Emission Max
520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
