Flow cytometric analysis of CD6 on human blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD6 (Cat. No. 555357; solid line histogram) and FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram). Fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of CD6 on human blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD6 (Cat. No. 555357; solid line histogram) and FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram). Fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
製品詳細
BD Pharmingen™
T12; TP120
Human (QC Testing)
Mouse BALB/c IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
IV T148
AB_395760
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 mL
カタログ番号
349202
Lysing Buffer RUO
サイズ
100 mL
カタログ番号
555899
FITC Mouse IgG1, κ Isotype Control RUO
サイズ
100 Tests
カタログ番号
555748
555357 Rev. 10
抗体の詳細
M-T605
The M-T605 monoclonal antibody specifically binds to CD6. CD6 is a 100-130 kDa type I transmembrane glycoprotein present on more than 80% of mature T cells and a subset of B cells. CD6 is also weakly expressed on cortical thymocytes. CD6 binds to CD166 and reportedly plays roles in cellular adhesion, costimulation of T lymphocyte proliferation, and the regulation of lymphocyte apoptosis. The antibody is useful for investigation of CD6+ B-cell function.
555357 Rev. 10
フォーマットの詳細
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
555357 Rev.10
引用＆参考文献
Development References (7)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Bott CM, Doshi JB, Li LL, McMurtry SA, Sanders JL, Fox DA. Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester. J Immunol. 1994; 153(1):1-9. (Biology). View Reference
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Endres N, Riethmuller G, Rieber EP. Functional characterization of a novel B-cell subsetdefined by the CD6 antigen. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:340.
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Rasmussen RA, Counts SL, Daley JF, Schlossman SF. Isolation and characterization of CD6- T cells from peripheral blood. J Immunol. 1994; 152(2):527-536. (Biology). View Reference
-
Reiter C. Cluster report: CD6. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:339.
-
Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
555357 Rev. 10
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
