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      FITC Mouse Anti-Human CD49b
      FITC Mouse Anti-Human CD49b
      Flow cytometric analysis of CD49B expression on human peripheral blood platelets. Human platelets were stained with FITC Mouse Anti-Human CD49b (Cat. No. 555498; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™ system.
      FITC Mouse Anti-Human CD49b
      Flow cytometric analysis of CD49B expression on human peripheral blood platelets. Human platelets were stained with FITC Mouse Anti-Human CD49b (Cat. No. 555498; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™ system.
      製品詳細
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      BD Pharmingen™
      Integrin alpha-2; ITGA2; VLA-2 alpha; Platelet GPIa; Collagen receptor; BR
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested)
      20 µl
      V S190
      AB_395888
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555498 Rev. 12
      抗体の詳細
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      AK-7

      The AK-7 monoclonal antibody specifically binds to CD49b. CD49b is also known as integrin α2 chain, VLA-α2, and GP1a. CD49b is a type I transmembrane glycoprotein and belongs to the integrin family of extracellular matrix and cell-cell adhesion receptors. CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b noncovalently associates with CD29 to form the VLA-2 complex (integrin α2/β1). The VLA-2 complex serves as a receptor for collagen and laminin. It can regulate responses mediated by proinflammatory effector cells.

      555498 Rev. 12
      フォーマットの詳細
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      555498 Rev.12
      引用＆参考文献
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      Development References (3)

      1. Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400. (Biology). View Reference
      2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      555498 Rev. 12

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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