BB515 Rat Anti-Integrin β7

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BD Horizon™ BB515 Rat Anti-Integrin β7

Name: BB515 Rat Anti-Integrin β7
Brand: BD Horizon™
SKU: 565331

クローン FIB504

(RUO)

Flow cytometric analysis of Integrin β7 expression on human peripheral blood lymphocytes. Whole blood was stained with either BD Horizon™ BB515 Rat IgG2a, κ Isotype Control (Cat. No. 564418; dashed line histogram) or BD Horizon BB515 Rat Anti-Human Integrin β7 antibody (Cat. No. 565331; solid line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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製品詳細

Brand: BD Horizon™

Alternative Name: Itgb7; Integrin β7; Integrin beta 7; ITB7; Ly-69; Ly69

Reactivity: Human (QC Testing), Mouse (Tested in Development)

Isotype: Rat F344, also known as Fischer, CDF IgG2a, κ

Immunogen: Mouse T lymphoma line TK1

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Workshop Number: VI A024, VI 6T-101

RRID: AB_2739188

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BB515 under optimum conditions and unconjugated antibody was removed.

推奨アッセイ手順

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

For optimal results, it is recommended to perform 2 washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescence staining, prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. Diagnostic uses require a separate license.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Brilliant Stain Buffer RUO

サイズ 100 Tests カタログ番号 563794

Lysing Solution 10X Concentrate RUO (GMP)

サイズ 100 mL カタログ番号 349202

Lysing Buffer RUO

サイズ 100 mL カタログ番号 555899

BB515 Rat IgG2a, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 564418

詳細を表示

565331 Rev. 2

抗体の詳細

FIB504

The FIB504 monoclonal antibody specifically recognizes mouse integrin β7 subunit (130 kDa) but also crossreacts with human integrin β7. Integrin β7 associates with α4 (CD49d) expressed on subsets of lymphocytes and thymocytes. It also associates with αIEL (CD103) expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Integrin β7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leucocytes to extravascular spaces during the inflammatory response.

The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filter. This dye has been exclusively developed by BD Biosciences and is up to seven times brighter than FITC with less spillover into the PE channel. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. It is not recommended to use BB515 in cocktails that include Streptavidin conjugates as it may cause high background.

565331 Rev. 2

フォーマットの詳細

BB515

The BD Horizon Brilliant™ Blue 515 (BB515) dye is part of the BD Horizon Brilliant™ Blue family of dyes. This dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 490-nm and an emission maximum (Em Max) of 515-nm. Driven by BD innovation, BB515 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 520-nm (e.g., 530/30-nm). BB515 reagents are significantly brighter than equivalent FITC or Alexa Fluor™ 488 reagents with less spillover into the PE detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BB515

励起源: Blue 488 nm

最大励起波長: 490 nm

最大蛍光波長: 515 nm

565331 Rev.2

引用＆参考文献

Development References (7)

Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Immunogen: Blocking, Flow cytometry, Functional assay). View Reference
Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Biology). View Reference
Butcher EC, Picker LJ. Lymphocyte homing and homeostasis. Science. 1996; 272(5258):60-66. (Biology). View Reference
Erle DJ, Briskin MJ, Butcher EC, Garcia-Pardo A, Lazarovits AI, Tidswell M. Expression and function of the MAdCAM-1 receptor, integrin alpha 4 beta 7, on human leukocytes. J Immunol. 1994; 153(2):517-528. (Biology). View Reference
Rott LS, Briskin MJ, Andrew DP, Berg EL, Butcher EC. A fundamental subdivision of circulating lymphocytes defined by adhesion to mucosal addressin cell adhesion molecule-1. Comparison with vascular cell adhesion molecule-1 and correlation with beta 7 integrins and memory differentiation. J Immunol. 1996; 156(10):3727-3736. (Clone-specific: Blocking, Flow cytometry, Functional assay). View Reference
Teague TK, Lazarovits AI, McIntyre BW. Integrin alpha 4 beta 7 co-stimulation of human peripheral blood T cell proliferation. Cell Adhes Commun. 1994; 2(6):539-547. (Biology). View Reference
Wimazal F, Agis H, Baghestanian M, Hagen W, Lechner K, Valent P. Endothelial Cell functional studies: Evaluation of mast cells and basophils with monoclonal antibodies against surface determinants related to vascular cells and adhesion. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:783-784.

すべて表示する (7)

565331 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.