Flow cytometric analysis of Alexa Fluor® 647 Mouse IgM, κ Isotype Control on blood lymphocytes. Whole blood was stained with Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; solid line histogram) or unstained (dashed line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). Fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSRII™.
Flow cytometric analysis of Alexa Fluor® 647 Mouse IgM, κ Isotype Control on blood lymphocytes. Whole blood was stained with Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; solid line histogram) or unstained (dashed line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). Fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSRII™.
製品詳細
BD Pharmingen™
Anti-TNP
Mouse BALB/c IgM, κ
TNP-Keyhole Limpet Hemocyanin
Flow cytometry, Isotype control (Routinely Tested)
0.2 mg/ml
AB_2034030
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 mL
カタログ番号
349202
Lysing Buffer RUO
サイズ
100 mL
カタログ番号
555899
560806 Rev. 2
抗体の詳細
G155-228
Alexa Fluor® 647 Mouse IgM, κ Isotype Control is specific for the hapten trinitrophenol (TNP), a hapten not expressed on human or non-human primate primary cells or cell lines. In the absence of specific binding, this immunoglobulin may bind non-specifically to Fc receptors.
560806 Rev. 2
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
560806 Rev.2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
