Flow cytometric analysis of CCL17 expression in mature human monocyte-derived dendritic cells. Monocyte-derived dendritic cells were generated from peripheral blood monocytes that were cultured (5 days) in complete RPMI medium containing recombinant human GM-CSF (Cat. No.550068) and IL-4 (Cat. No. 554605) proteins and restimulated (2 days) with lipopolysaccharide. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714), and then stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CCL17 antibody (Cat. No. 566621; solid line histogram) at 0.5 ug/test. The histogram showing expression of human CCL17 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocyte-derived dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on the Technical Data Sheet are not lot specific.
Flow cytometric analysis of CCL17 expression in mature human monocyte-derived dendritic cells. Monocyte-derived dendritic cells were generated from peripheral blood monocytes that were cultured (5 days) in complete RPMI medium containing recombinant human GM-CSF (Cat. No.550068) and IL-4 (Cat. No. 554605) proteins and restimulated (2 days) with lipopolysaccharide. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714), and then stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CCL17 antibody (Cat. No. 566621; solid line histogram) at 0.5 ug/test. The histogram showing expression of human CCL17 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocyte-derived dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on the Technical Data Sheet are not lot specific.
製品詳細
BD Pharmingen™
CCL17; C-C motif chemokine 17; ABCD-2; SCYA17; TARC; A-152E5.3
Human (QC Testing)
Mouse IgG1, κ
Human CCL17 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869797
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Fixation/Permeabilization Kit RUO
サイズ
250 Tests
カタログ番号
554714
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
サイズ
50 µg
カタログ番号
565571
566621 Rev. 1
抗体の詳細
T48-854
The T48-854 monoclonal antibody reacts with C-C motif chemokine 17 (CCL17), which is also known as Thymus and Activation-Regulated Chemokine (TARC), or Small-inducible cytokine A17 (SCYA17). CCL17 is produced by activated T cells, monocytes, or dendritic cells. It is constitutively produced by thymic stromal cells. CCL17 binds to and signals through cell surface C-C chemokine receptor type 4 (CCR4). CCR4 is expressed on NK cells, basophils, macrophages, and T cells, such as, Th2-like cells, skin-homing T cells, and some regulatory T cells. CCL17 plays a role in T cell development and is involved in the activation and trafficking of leucocytes, including effector T cells which mediate peripheral immune responses.
566621 Rev. 1
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566621 Rev.1
引用＆参考文献
Development References (3)
-
Bernardini G, Hedrick J, Sozzani S. Identification of the CC chemokines TARC and macrophage inflammatory protein-1 beta as novel functional ligands for the CCR8 receptor. J Immunol. 1998; 28(2):582-588. (Biology). View Reference
-
D'Ambrosio D, Iellem A, Bonecchi R, et al. Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells.. J Immunol. 1998; 161(10):5111-5. (Biology). View Reference
-
Imai T, Yoshida T, Baba M, Nishimura M, Kakizaki M, Yoshie O. Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector.. J Biol Chem. 1996; 271(35):21514-21. (Biology). View Reference
566621 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
