HeLa + Anisomycin Cell Lysate
- Brand BD Transduction Laboratories™
- Concentration 1.0 mg/ml
Western blot (Routinely Tested)
- Storage Buffer SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)
- Regulatory Status RUO
Regulatory Status Legend
Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. HeLa (human epitheloid carcinoma) cells, were grown to 80-90% confluency, then treated with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 minutes at 37°C. Anisomycin has been established as a potent activator of the p38/JNK MAPK pathway.
Preparation and Storage
Store undiluted at -20°C.
Do not dilute or boil.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.