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Alexa Fluor® 647 Rat Anti-Blimp-1
Alexa Fluor® 647 Rat Anti-Blimp-1
Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon™ BV786 Mouse Anti-Human CD27 (Cat No. 563327) and BD Horizon BUV737 Mouse Anti-Human CD19 (Cat. No. 564303) antibodies, washed, and fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725). The cells were then stained with Alexa Fluor® 647 Rat Anti-Blimp-1 antibody (Cat. No. 565002). Histograms (Right Panel) showing Blimp-1 expression were derived from either CD19+CD27- (gray shaded histogram), CD19+CD27+ (dashed line histogram), or CD19loCD27+ (black solid line histogram) gated events as indicated by the two-color contour plot (Left Panel) showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon™ BV786 Mouse Anti-Human CD27 (Cat No. 563327) and BD Horizon BUV737 Mouse Anti-Human CD19 (Cat. No. 564303) antibodies, washed, and fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725). The cells were then stained with Alexa Fluor® 647 Rat Anti-Blimp-1 antibody (Cat. No. 565002). Histograms (Right Panel) showing Blimp-1 expression were derived from either CD19+CD27- (gray shaded histogram), CD19+CD27+ (dashed line histogram), or CD19loCD27+ (black solid line histogram) gated events as indicated by the two-color contour plot (Left Panel) showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
製品詳細
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BD Pharmingen™
BLIMP-1; BLIMP1; PRDM1; PR domain containing 1, with ZNF domain; PRDI-BF1
Human (QC Testing), Mouse (Tested in Development)
Rat IgG2a, κ
Mouse Blimp-1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739040
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

推奨アッセイ手順

Clone 6D3 crossreacts with human and mouse Blimp-1. Customers could also consider fluorescent conjugates of clone 5E7 (Rat Anti-Mouse

Blimp-1) for the staining and flow cytometric analysis of mouse cells that express Blimp-1.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  9. An isotype control should be used at the same concentration as the antibody of interest.
565002 Rev. 1
抗体の詳細
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6D3

The 6D3 monoclonal antibody specifically binds to mouse B lymphocyte-induced maturation protein 1 (Blimp-1) and crossreacts with human BLIMP-1. Blimp-1 is a 98 kDa zinc finger-containing protein that is encoded by the Prdm1 gene. Blimp-1 was first discovered as a transcriptional repressor of the IFN-β promoter. Blimp-1 is critical for primordial germ cells specification in the early embryo and induction of terminal cell differentiation in multiple somatic cell types. It is largely known as a master regulator of antibody-secreting plasma cells due to its function in the repression of PAX5, CIITA and cMYC genes. Blimp-1 also controls T cell homeostasis and effector differentiation through different mechanisms, including repression of IL-17 producing cells.  Blimp-1 also plays roles in the maturation and activity of dendritic cells and natural killer cells.

565002 Rev. 1
フォーマットの詳細
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565002 Rev.1
引用&参考文献
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Development References (16)

  1. Chan YH, Chiang MF, Tsai YC, et al. Absence of the transcriptional repressor Blimp-1 in hematopoietic lineages reveals its role in dendritic cell homeostatic development and function. J Immunol. 2009; 183(11):7039-7046. (Biology). View Reference
  2. Crotty S, Johnston RJ, Schoenberger SP. Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation. Nat Immunol. 2010; 11(2):114-120. (Biology). View Reference
  3. Huang S. Blimp-1 is the murine homolog of the human transcriptional repressor PRDI-BF1. Cell. 1994; 78(1):9. (Biology). View Reference
  4. Johnston RJ, Poholek AC, DiToro D, et al. Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.. Science. 2009; 325(5943):1006-10. (Biology). View Reference
  5. Jourdan M, Caraux A, De Vos J, et al. An in vitro model of differentiation of memory B cells intoplasmablasts and plasma cells including detailed phenotypic and molecularcharacterizatio. Blood. 2009; 114(25):5173-5181. (Methodology: Cell differentiation). View Reference
  6. Kallies A, Carotta S, Huntington ND, et al. A role for Blimp1 in the transcriptional network controlling natural killer cell maturation. Blood. 2011; 117(6):1869-1879. (Biology). View Reference
  7. Kallies A, Hasbold J, Tarlinton DM, et al. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med. 2004; 200(8):967-977. (Immunogen: Western blot). View Reference
  8. Kallies A, Hawkins ED, Belz GT, et al. Transcriptional repressor Blimp-1 is essential for T cell homeostasis and self-tolerance. Nat Immunol. 2006; 7(5):466-474. (Biology). View Reference
  9. Keller AD, Maniatis T. Identification and characterization of a novel repressor of beta-interferon gene expression. Genes Dev. 1991; 5(5):868-879. (Biology). View Reference
  10. Kim SJ, Gregersen PK, Diamond B. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest. 2013; 123(2):823-833. (Biology). View Reference
  11. Martins GA, Cimmino L, Liao J, Magnusdottir E, Calame K. Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival. J Exp Med. 2008; 205(9):1959-1965. (Biology). View Reference
  12. Ohinata Y, Payer B, O'Carroll D, et al. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature. 2005; 436(7048):207-213. (Biology). View Reference
  13. Salehi S, Bankoti R, Benevides L, et al. B lymphocyte-induced maturation protein-1 contributes to intestinal mucosa homeostasis by limiting the number of IL-17-producing CD4+ T cells. J Immunol. 2012; 189(12):5682-5693. (Biology). View Reference
  14. Shaffer AL, Lin KI, Kuo TC, et al. Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program. Immunity. 2002; 17(1):51-62. (Biology). View Reference
  15. Smith MA, Maurin M, Cho HI, et al. PRDM1/Blimp-1 controls effector cytokine production in human NK cells.. J Immunol. 2010; 185(10):6058-67. (Biology). View Reference
  16. Turner CA Jr, Mack DH, Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells. Cell. 1994; 77(2):297-306. (Biology). View Reference
すべて表示する (16) 表示項目を減らす
565002 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.