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FITC Mouse Anti-EGF Receptor 13/EGFR RUO

FITC Mouse Anti-EGF Receptor 

Clone  13/EGFR   (RUO)

  • Brand BD Transduction Laboratories™
  • Concentration 250 µg/ml
  • Isotype Mouse IgG1
  • Reactivity Human (QC Testing)  Mouse (Tested in Development) 
  • Application Immunofluorescence (Routinely Tested) 
  • Immunogen Human EGF Receptor aa. 1020-1046
  • Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cγ. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos.

This antibody is routinely tested by immunofluorescence. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

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Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


  1. Carpenter G. Receptors for epidermal growth factor and other polypeptide mitogens. Annu Rev Biochem. 1987; 56:881-914. View reference

  2. Golubovskaya V, Beviglia L, Xu LH, Earp HS 3rd, Craven R, Cance W. Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells. J Biol Chem. 2002; 277(41):38978-38987. View reference

  3. Ward CW, Gough KH, Rashke M, Wan SS, Tribbick G, Wang J. . Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor.. J Biol Chem. 1996; 271(10):5603-5609. View reference

  4. Wu TT, Castle JD. Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor. Mol Biol Cell. 1998; 9(7):1661-1674. View reference

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