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      Purified Mouse Anti-iNOS/NOS Type II
      Purified Mouse Anti-iNOS/NOS Type II
      Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- iNOS/NOS Type II antibody.
      Purified Mouse Anti-iNOS/NOS Type II
      Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS.
      Purified Mouse Anti-iNOS/NOS Type II Purified Mouse Anti-iNOS/NOS Type II
      Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- iNOS/NOS Type II antibody.
      Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS.
      製品詳細
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      BD Transduction Laboratories™
      Mouse (QC Testing)
      Mouse IgG2a
      Mouse iNOS aa. 961-1144
      Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
      130 kDa
      250 µg/ml
      AB_397718
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      610328 Rev. 2
      抗体の詳細
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      6/iNOS/NOS Type II

      Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-γ, TNF-α, and IL-1) and microbial products (lipopolysaccharide (LPS)), iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFκB. Data indicates that TGF-β affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.

      610328 Rev. 2
      フォーマットの詳細
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610328 Rev.2
      引用＆参考文献
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      Development References (5)

      1. Heneka MT, Klockgether T, Feinstein DL. Peroxisome proliferator-activated receptor-gamma ligands reduce neuronal inducible nitric oxide synthase expression and cell death in vivo. J Neurosci. 2000; 20(18):6862-6867. (Biology: Immunohistochemistry, Western blot). View Reference
      2. Marrogi A, Pass HI, Khan M, Metheny-Barlow LJ, Harris CC, Gerwin BI. Human mesothelioma samples overexpress both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (NOS2): in vitro antiproliferative effects of a COX-2 inhibitor. Cancer Res. 2000; 20(18):6862-6867. (Biology: Immunofluorescence, Immunohistochemistry). View Reference
      3. Miles PR, Bowman L, Rao KM, Baatz JE, Huffman L. Pulmonary surfactant inhibits LPS-induced nitric oxide production by alveolar macrophages. Am J Physiol. 1999; 276(1):L186-L196. (Biology: Western blot). View Reference
      4. Sciorati C, Rovere P, Ferrarini M, Heltai S, Manfredi AA, Clementi E. Autocrine nitric oxide modulates CD95-induced apoptosis in gammadelta T lymphocytes. J Biol Chem. 1997; 272(37):23211-23215. (Biology: Flow cytometry, Western blot). View Reference
      5. Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). View Reference
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      610328 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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